Figure 1.

AR and SET9 interact in vitro and in vivo. (A) LNCaP cells grown in steroid-depleted media and treated with and without 10 nM DHT for 6 h were subject to IP using an anti-SET9 antibody followed by western analysis using an anti-AR antibody. (B) HEK293T cells were transiently transfected with pFlag-AR and either pFlag-SET9 or pFlag-SET9_H297A for 48 h prior to IP using an anti-AR antibody followed by immunoblotting with anti-AR and -Flag antibodies. (C) Diagrammatic representation of the domains of the androgen receptor showing target lysine sequences of SET9 and sequence of AR peptide used in ³H methylation assays. (D) In vitro IP between His-tagged SET9 and N- and C-terminal fragments of the AR using anti-SET9 antibody followed by western analysis using an anti-His antibody.