Figure 4.
SET9 is an AR co-activator. (A) LNCaP cells grown in steroid-depleted media were transiently transfected with non-silencing (N/S) or SET9 siRNAs for 66 h prior to treatment with and without 10 nM DHT for 6 h followed by RNA extraction and real-time PCR analysis of PSA and TMPRSS2 mRNA expression. (B) HEK293T and LNCaP cells were transiently transfected for 48 h with AR (HEK293T cells only) and either SET9 or SET9H297A together with both MMTV-luciferase and β-galactosidase reporters prior to luciferase and β-galactosidase expression analyses. (C) HEK293T cells were transiently transfected and analysed as in (B) with the inclusion of ARK632R. Data represent average of three independent repeats ± standard error (A–C).