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. 2010 Oct 14;39(4):1243–1255. doi: 10.1093/nar/gkq896

Figure 3.

Figure 3.

Expression of myogenin and Mef2D1b complements the loss of myogenic miRNA expression in a Prmt5 AS cell line. (A and B) Relative expression of myogenin and Mef2D1b upon differentiation in NIH 3T3 and in the Prmt5 AS cell line, C1, infected with retrovirus encoding myogenin and Mef2D1b. (C–F) qPCR analyses of primary transcripts of miR-1 and miR-133a in NIH 3T3 and C1 cells expressing myogenin and Mef2D1b at various times post-differentiation. The data represent the average of three independent experiments ± standard deviation. Expression at Time 0 in the empty vector (EV) control is normalized to 1. h, hours.