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. 2010 Oct 14;39(4):1243–1255. doi: 10.1093/nar/gkq896

Figure 4.

Figure 4.

Carm1/Prmt4 is required for the induction of myogenic miRNA expression during myogenesis. (A) Immunoblot demonstrating the absence of Carm1/Prmt4 in the knockout (KO) cells. A cross-reacting band (asterisk) demonstrates equal loading between gel lanes. (B–D) Relative expression of MyoD, myogenin and Acta1 mRNAs in the wild-type (WT) and Carm1/Prmt4 KO cells differentiated along the myogenic pathway by expressing MyoD or the empty vector (EV) control. (E–H) Relative expression of primary transcripts of miR-1-1, miR-1-2, miR-133a-1 and miR-133a-2 at various times post-differentiation in the WT and Carm1/Prmt4 knockout (KO) cells. (I and J) Relative expression of Mef2D and SRF mRNAs at various times post-differentiation in the WT and Carm1/Prmt4 knockout (KO) cells. The data represent the average of three independent experiments ± standard deviation. The expression at time 0 in empty vector infected cells was normalized to 1. h, hours.