Figure 8.

The myogenic regulatory factors MyoD and myogenin and the chromatin remodeling enzyme Brg1 interact with the proximal E-boxes containing sequences upstream of miR-1-1. ChIP experiments demonstrated the binding of MyoD, myogenin and Brg1 at regions containing the proximal E-boxes (A–C) but not to the distal E-boxes (D and E) of miR-1-1 at the indicated times in MyoD differentiated WT and Carm1/Prmt4 KO cells. Values at time 0 in the empty vector (EV) control were normalized to 1. The experiment was repeated three times, and the data represent the average of three experiments ± standard deviation. h, hours.