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(A) Library of non-natural nucleotides used to interrogate the mechanism accounting for the preferential utilization of dATP during the replication of an abasic site. For clarity, only the nucleobases of the 5-substituted indolyl nucleotides are shown. (B) SAR highlighting the importance of π-electron surface area of the incoming nucleotide for efficient incorporation opposite an abasic site. See text for details.
