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. 2010 Oct 18;39(4):1256–1265. doi: 10.1093/nar/gkq926

Figure 5.

Figure 5.

(A) ChIP analysis to determine the binding of nucleolin to the VEGF promoter region containing the polypurine/polypyrimidine tract in A498 renal carcinoma cells. Recruitment of RNA Polymerase II (Pol II) (lanes 3), Sp1 (lanes 4) and nucleolin (lanes 5) to the VEGF proximal promoter was assessed using primers specific to the VEGF promoter (–248 to +48). One percent of total input DNA was used as a loading control (lane 1) and isotype-matched IgG was used as an internal control for the immunoprecipitation (lane 2). (B) PCR amplification of immunoprecipitated DNAs using primers specific to the 5′ upstream promoter region (–1079 to –874) of the VEGF gene as a negative control. (C) PCR amplification of immunoprecipitated DNAs using primers specific to the proximal promoter region (−273 to +71) of the HIF−1α gene as a positive control. Data shown are representative of at least two experiments.