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9S ribosomal RNA precursor processing by C. crescentus and E. coli RNase E catalytic domain. Digestion time points (0, 15 min) were analysed by denaturing polyacrylamide gel and detected with SYBR Gold stain. The p5S product, as seen to be released by both RNase E enzymes, is a precursor of the mature 120 nt 5S rRNA. The length of RNA species were determined by comparing to RiboRuler™ low-range RNA marker (Fermentas), in the leftmost lane.
