Table 2.
Dynamics of LEDGF/p75 PWWP mutants measured with sFRAP and FCS
| eGFP-LEDGF/p75 K56D |
eGFP-LEDGF/p75 R74D |
eGFP-LEDGF/p75 K56D R74D |
||||
|---|---|---|---|---|---|---|
| sFRAP | FCS | sFRAP | FCS | sFRAP | FCS | |
| Fimm (%) | −0.1 ± 0.1 | 0.1 ± 0.1 | −0.1 ± 0.1 | |||
| Dfast (µm2/s) | – | 16.4 ± 3.5 | – | 13.3 ± 2.4 | – | 16.8 ± 5.1 |
| Ffast (%) | – | 44.5 ± 9.3 | – | 58.5 ± 8.6 | – | 62.8 ± 13.8 |
| Dslow (µm2/s) | 1.97 ± 0.09 | 1.0 ± 0.3 | 3.02 ± 0.18 | 1.4 ± 0.6 | 3.25 ± 0.20 | 1.5 ± 0.8 |
| α | – | 0.69 ± 0.05a | – | 0.78 ± 0.04 | – | 0.80 ± 0.05 |
Values represent averages of at least 10 (sFRAP) or 20 (FCS) measurements and are ±SD. Parameters Dfast, Ffast and Dslow are obtained from a two-component normal diffusion fit. Parameter α is obtained from a one-component anomalous fit.
aFit not satisfying. For more details on the parameters, see Table 1.