Skip to main content
. 2010 Oct 20;39(4):1565–1575. doi: 10.1093/nar/gkq974

Figure 6.

Figure 6.

Mutations at the RNA-binding surface affect RIG-I signaling. (A) Effects of structures of the RNA ligands on the activation of IFN-β luciferase production. The assay shows the activities of a 27-nt 5′ ppp ssRNA (3PssR27), a 24-bp 5′ ppp dsRNA (3PdsR24) and a 27-bp blunt-ended dsRNA (dsR27) in stimulating RIG-I signaling in HEK 293T cells. The error bars correspond to the standard deviations of signals from three independent transfections. (B) Effects of mutations at the RNA-binding surface on IFN-β luciferase production in cells transfected with a 27-bp blunt-ended dsRNA and a 24-bp dsRNA with 5′ ppp. Expression of the wild-type and mutants of RIG-I in the transfected cells were confirmed by western blot (inset).