Table 1.
Sequences of the translation initiation signals in pOD vectors
| Vector | Description | Sequence |
|---|---|---|
| pOD1 | Reference construct | GGATCCAAATACTGCAGTTTAACTTTAAGAAGGAGATATACCCATGG |
| pOD2 | Two spaced SD-sequences | GGATCCAAATACTGCAGCTTAGAATATTGAAGGAGATATACCCATCTATTATAAATAGTGCAGTTTAACTTTAA GAAGGAGATATACCCATGG |
| pOD3 | Three spaced SD-sequences | GGATCCAAATACTGCAGAGATACAATAAGAAGGAGATATACCCATCTGTATTAAATAGTGCAGCTTAGAATATT GAAGGAGATATACCCATCTATTATAAATAGTGCAGTTTAACTTTAAGAAGGAGATATACCCATGG |
| pOD4 | One start-stop without spacer | GGATCCAAATACTGCAGTTTAACTTTAAGAAGGAGATATACATATGTAATAAGAAGGAGATATACCCATGG |
| pOD5 | Two start-stop without spacers | GGATCCAAATACTGCAGTTTAACTTTAAGAAGGAGATATACATATGTAGTAAGAAGGAGATATACATATGTAATAA GAAGGAGATATACCCATGG |
| pOD6 | Two SD-sequences without spacer | GGATCCAAATACTGCAGTTTAACTTTAAGAAGGAGATAGAAGGAGATATACCCATGG |
| pOD7 | Three SD-sequences without spacers | GGATCCAAATACTGCAGTTTAACTTTAAGAAGGAGATAGAAGGAGATAGAAGGAGATATACCCATGG |
| pOD8 | One mini-ORF without spacer | GGATCCAAATACTGCAGTTTAACTTTAAGAAGGAGATATACATATGGGTTAATAAGAAGGAGATATACCCATGG |
| pOD9 | Two mini-ORFs without spacers | GGATCCAAATACTGCAGTTTAACTTTAAGAAGGAGATATACATATGGGATAGTAAGAAGGAGATATACATATGGGTTAATAAGAAGGAGATATACCCATGG |
| pOD11 | Two non-spaced translation initiation sites; peptidase cleavage site | GGATCCAAATACTGCAGTTTAACTTTAAGAAGGAGATATACATATGGGAGAAGGAGATATACCCATGggtgaaaatttatattttcaaagcatgg |
| pOD12 | Three non-spaced translation initiation sites; peptidase cleavage site | GGATCCAAATACTGCAGTTTAACTTTAAGAAGGAGATATACATATGGGAGAAGGAGATATACATATGGGA GAAGGAGATATACCCATGggtgaaaatttatattttcaaagcatgg |
| pOD13 | Reference construct; peptidase cleavage site | GGATCCAAATACTGCAGTTTATCTTTATGAAGGAGATATACCCATGggtgaaaatttatattttcaaagcatgg |
| pOD14 | Two spaced translation initiation sites; peptidase cleavage site | GGATCCAAATACTGCAGCTTACAATATTGAAGGAGATATACATATGGGTATTATAAATAGTGCAGTTTATCTTTAT GAAGGAGATATACCCATGggtgaaaatttatattttcaaagcatgg |
| pOD15 | Three spaced translation initiation sites; peptidase cleavage site | GGATCCAAATACTGCAGCTTACAATATTGAAGGAGATATACATATGGGTATTATAAATAGTGCAGCTTACAATATT GAAGGAGATATACATATGGGTATTATAAATAGTGCAGTTTATCTTTATGAAGGAGATATACCCATGggtgaaaatttatattttcaaagcatgg |
| pOD17 | Two non-spaced translation initiation sites; peptidase cleavage site | GGATCCAAATACTGCAGTTTAACTTTAAGAAGGAGATATACATATGGGAGAAGGAGATATACCCATGG |
| pOD18 | Three non-spaced translation initiation sites; peptidase cleavage site | GGATCCAAATACTGCAGTTTAACTTTAAGAAGGAGATATACATATGGGAGAAGGAGATATACATATGGGA GAAGGAGATATACCCATGG |
| pOD19 | Reference construct without peptidase cleavage site | GGATCCAAATACTGCAGTTTATCTTTATGAAGGAGATATACCCATGG |
| pOD20 | Two spaced translation initiation sites | GGATCCAAATACTGCAGCTTACAATATTGAAGGAGATATACATATGGGTATTATAAATAGTGCAGTTTATCTTTAT GAAGGAGATATACCCATGG |
| pOD21 | Three spaced translation initiation sites | GGATCCAAATACTGCAGCTTACAATATTGAAGGAGATATACATATGGGTATTATAAATAGTGCAGCTTACAATATT GAAGGAGATATACATATGGGTATTATAAATAGTGCAGTTTATCTTTATGAAGGAGATATACCCATGG |
| pOD22 | Four spaced translation initiation sites; peptidase cleavage site | GGATCCAAATACTGCAGCTTACAATATTGAAGGAGATATACATATGGGTATTATAAATAGTGCAGCTTACAATATT GAAGGAGATATACATATGGGTATTATAAATAGTGCAGCTTACAATATTGAAGGAGATATACATGGTATTATAAATAGTGCAGTTTATCTTTATGAAGGAGATATACCCATGggtgaaaatttatattttcaaagcatgg |
Italic letters indicate restriction sites (BamHI, PstI and NcoI). Underlined letters indicate SD-sequences. Bold letters indicate start codons, stop codons and mini-ORFs. Lower case letters indicate TEV peptidase cleavage site.