Table 3.

Utilization of SD sequences in constructs harboring multiple ribosome-binding sites

Construct	Initiation from SD No. (3′→5′)	Fraction of GFP accumulation
		Escherichia coli (%)	Nicotiana tabacum plastids (%)
pOD11	1	49.26	ND
	2	50.74	100
pOD12	1	40.41	5.78
	2	31.08	18.34
	3	28.52	75.88
pOD14	1	14.73	ND
	2	85.27	100
pOD15	1	15.57	ND
	2	51.98	55.93
	3	32.46	44.07
pOD17	1	56.14	40.01
	2	43.86	59.99
pOD18	1	49.11	4.43
	2	20.10	17.75
	3	30.80	77.82
pOD20	1	32.20	19.90
	2	67.80	80.10
pOD21	1	20.75	2.38
	2	46.49	71.26
	3	32.76	26.35
pOD22	1	11.71	–
	2	54.02	–
	3	11.28	–
	4	22.99	–

Data represent the means of at least two independently quantitated biological replicas. ND, not detectable; –, not analyzed.