Figure 2.

Binding of WT and mutant ROS1 proteins to substrate and product DNA. DNA-binding reactions were performed incubating increasing concentrations of WT ROS1 or mutant variants with 100 nM of fluorescein-labeled 5-meC:G substrate (upper panel), alexa-labeled homoduplex (center panel) or alexa-labeled 1-nt-gapped duplex product (lower panel). After nondenaturing gel electrophoresis, the gel was scanned to detect fluorescein- or alexa-labeled DNA. Protein–DNA complexes were identified by their retarded mobility compared with that of free DNA, as indicated. The fraction of bound DNA is indicated below each lane. The asterisk depicts 5′-end labeling of the upper strand. M: 5-meC.