Table 1.
Overview of binding constants of examined protein variants as determined by analytical ultracentrifugation
| Protein | Binding constant (KA) |
|---|---|
| χ wild-type | (2.9 ± 1) × 105 M−1 |
| χ V117F | ND |
| χ V117I | ND |
| χ Y119A | (3.4 ± 1) × 105 M−1 |
| χ K124A | (2.1 ± 0.5) × 104 M−1 |
| χ K124M | (6 ± 1) × 104 M−1 |
| χ R128A | ND |
| χ Y131A | (4.8 ± 1) × 104 M−1 |
| χ Y131L | ND |
| χ K132A | (9 ± 2.5) × 104 M−1 |
| χ K132M | (2.1 ± 1) × 105 M−1 |
| SSB+Gly | ND |
In the case of the V117F/I, R128A and Y131L mutants of χ and the C-terminal extension mutant of SSB, SSB+Gly, the binding affinity was so weak that no binding constant could be determined (ND).