Table 2.
IC50 Values of 1 – 14, M-1 – M-4, and A-1 – A-3 suppressing HIF-1 Activation in a T47D Cell-based Reporter Assay.
| compound | 1% O2, 16 h | 10 μM 1,10-phen, 16 h | ||
|---|---|---|---|---|
| IC50 μM | (95% CI) μM | IC50 μM | (95% CI) μM | |
| 1 | 2.23 | (1.94 – 2.56) | 2.30 | (2.02 – 2.62) |
| 2 | 1.72 | (1.52 – 1.93) | 1.81 | (1.46 – 2.24) |
| 3 | 4.19 | (3.83 – 4.57) | 4.12 | (3.46 – 4.90) |
| 4 | 5.70 | (5.23 – 6.20) | 5.31 | (4.68 – 6.03) |
| 5 | 2.76 | (2.53 – 3.00) | 2.71 | (2.42 – 3.04) |
| 6 | 3.73 | (3.45 – 4.03) | 3.40 | (2.97 – 3.90) |
| 7 | 9.40 | (8.50 – 10.4) | 9.56 | (8.54 – 10.71) |
| 8 | 9.96 | (8.75 – 11.3) | 8.21 | (7.29 – 9.26) |
| 9 | 2.20 | (1.79 – 2.71) | 1.28 | (0.96 – 1.70) |
| 10 | 5.04 | (4.16 – 6.11) | 3.25 | (2.80 – 3.77) |
| 11 | 4.44 | (3.82 – 5.16) | 3.71 | (2.99 – 4.60) |
| 12 | 4.63 | (3.92 – 5.46) | 4.82 | (4.20 – 5.54) |
| 13 | 3.23 | (2.95 – 3.54) | 3.73 | (3.03 – 4.59) |
| 14 | 6.76 | (6.31 – 7.25) | 5.96 | (5.30 – 6.71) |
| M-1 | 13.2 | (11.9 – 14.7) | 18.4 | (14.6 – 23.2) |
| M-2 | 5.91 | (5.26 – 6.65) | 15.7 | (13.4 – 18.4) |
| M-3 | 6.31 | (5.57 – 7.14) | 6.50 | (6.03 – 7.01) |
| M-4 | 7.42 | (6.04 – 9.11) | 11.3 | (10.2 – 12.6) |
| A-1 | 5.11 | (4.24 – 6.16) | 5.47 | (4.91 – 6.10) |
| A-2 | 0.83 | (0.80 – 0.86) | 1.06 | (0.98 – 1.15) |
| A-3 | 2.06 | (1.76 – 2.42) | 1.68 | (1.41 – 2.00) |
| 15 | 0.96 | (0.91 – 1.01) | 0.90 | (0.77 – 1.06) |
The IC50 and 95% CI values were determined from one representative of two independent experiments each performed in triplicate. The data on mammea E/BB (15) were from an earlier publication.10