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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Virus Res. 2010 Dec 31;156(1-2):147–150. doi: 10.1016/j.virusres.2010.12.014

Table 1.

Rescue efficiency, stock titers and plaque size of viruses.

Virus Rescue
efficiency
(log10PFU/ml)* 		Titer of stock virus
grown in MDCK
cells (log10PFU/ml)		 Plaque size
in MDCK
cells (mm)†
CA04 4.26 7.88 0.26 ± 0.08
k23 2.48 7.68 0.58 ± 0.22
k23HA-CA04 6.04 9.00 1.57 ± 0.35
CA04NA-k23 5.27 8.08 1.01 ± 0.24
CA04M-k23 4.26 7.72 0.91 ± 0.29
CA04NA,M-k23 6.00 8.73 1.41 ± 0.28
Nw3487 5.23 8.70 1.01 ± 0.36
k23HA-Nw3487 6.15 9.30 1.76 ± 0.31
Nw3487NA,M-k23 5.95 8.73 1.25 ± 0.34
Ut42 4.15 7.68 0.29 ± 0.09
k23HA-Ut42 6.08 9.08 1.42 ± 0.36
Ut42NA,M-k23 6.00 8.83 1.27 ± 0.31
*

Measured by determining the viral titer in the supernatant of transfected 293T cells at 48 hours post-infection, by plaque assay using MDCK cells.

†

Values represent mean ± standard deviations, measured at 48 hours post-infection; n=100. The plaque size of CA04NA,M-k23 differed significantly from that of CA04NA-k23 and CA04M-k23 (p < 0.001).