TABLE 1.
The function of IFM during ischemia with amobarbital or azide treatment
| Time control (n=9) | Ischemia (n=9) | Amobarbital + ischemia (n=9) | Azide + ischemia (n=5) | |
|---|---|---|---|---|
| Glutamate - Complex I substrate | ||||
| 0.3 mM DNP | 277±10 | 186±18* | 283±14 | 189±13* |
| TMPD-ascorbate – Complex IV substrate | ||||
| 0.3 mM DNP | 807±131 | 659±74 | 773±55 | 592±56 |
| Cytochrome c content | ||||
| (nmol/mg protein) | 0.276±0.016 | 0.255±0.018 | 0.301±0.016 | 0.264±0.012 |
Data are expressed as mean ± SEM;
*
p<0.05 vs. time control or amobarbital + ischemia.
Dinitrophenol (DNP) was used as an uncoupler to stimulate respiration.