Fig. 5.

Hepatocyte apoptosis induced by ischemia/reperfusion assessed by the TUNEL method. To assess the effect of the iNOS inhibitor on hepatocyte apoptosis, samples from mice treated with PBS or aminoguanidine (AG) prior to ischemia for 90 min were examined. Samples were obtained from (A) sham operated WT, (B) sham operated FL, and (C) FL mice undergoing 90 min ischemia and 4 h reperfusion (I/R) with PBS treatment, (D) WT mice undergoing I/R with PBS treatment, (E) FL mice undergoing I/R with AG treatment. (F) WT mice undergoing I/R with AG treatment. Original magnification ×200. Arrows indicate hepatocyte apoptosis. The apoptotic index was also calculated in mice 4 h after reperfusion (G). The data represent the mean ± SEM with n = 6 per group. *p<0.05 compared to mice treated with PBS.