Table 2.

Development of human hematopoietic lineages in bnx mice cotransplanted with CD34⁺ progenitors and human stroma expressing dual cytokines

Mouse #	Hu Stroma Producing:	%Hu CD45	%Hu CD4	%Hu CD8	%Hu CD33	%Hu CD19	%Hu 45
							SPLN	BLD
31	IL-3 + IL-7	0.4	0.3	0.2	0.0	0.0	0.0	0.0
32	IL-3 + IL-7	4.8	0.7	3.1	0.1	0.0	0.0	0.0
33	IL-3 + IL-7	4.1	1.4	2.3	0.1	0.0	0.0	0.0
34	IL-3 + IL-7	2.9	1.0	1.6	0.0	0.1	0.0	0.0
35	IL-3 + IL-7	5.6	2.7	2.7	0.0	0.2	0.0	3.6
36	IL-3 + IL-7	3.4	2.0	1.8	0.1	0.3	0.0	0.0
37	IL-3 + IL-7	0.3	0.0	0.1	0.0	0.0	0.0	0.0
	Ave.	3.1	1.2	1.7	0.0	0.0
38	IL-3 + SCF	10.7	2.8	3.6	2.0	0.0	1.2	4.9
39	IL-3 + SCF	5.3	2.7	1.2	1.4	0.0	2.4	3.3
40	IL-3 + SCF	3.0	0.9	1.5	1.1	0.0	0.9	0.0
41	IL-3 + SCF	0.6	0.0	0.0	0.4	0.0	0.0	0.0
42	IL-3 + SCF	2.2	0.8	0.7	0.9	0.0	0.0	0.0
	Ave.	4.4	1.4	1.4	1.2	0.0
43	IL-3 + FL	3.4	1.2	1.0	1.5	0.0	0.0	0.0
44	IL-3 + FL	0.7	0.2	0.0	0.6	0.0	0.0	0.0
45	IL-3 + FL	4.8	1.4	2.1	1.5	0.1	1.6	0.8
46	IL-3 + FL	2.6	0.7	0.4	1.8	0.0	0.0	0.0
	Ave.	2.7	0.9	0.9	1.4	0.0
47	IL-3	1.9	0.0	1.5	0.6	0.0	0.0	0.0
48	IL-3	3.1	0.1	2.0	1.4	0.0	0.0	0.0
49	IL-3	4.5	1.2	2.4	1.1	0.0	0.0	0.0
50	IL-3	3.0	1.0	0.9	0.7	0.0	1.5	0.0
51	IL-3	2.2	0.2	0.4	1.9	0.0	2.7	1.4
52	IL-3	4.9	1.5	0.8	2.2	0.1	0.0	0.9
	Ave.	3.4	0.7	1.3	1.3	0.0

Mice 31–46 were transplanted with 1 ×10⁵ IL-3-producing stromal cells and 9 ×10⁵ stromal cells engineered to produce FL, SCF or IL-7. Mice 47–52 received 1 × 10⁶ IL-3-producing stromal cells. All mice were cotransplanted with 1 × 10⁶ human progenitors. Mice were sacrificed six to eight months later, and the percentage of HuCD45⁺ cells in marrow, spleen and blood, plus the percentage of human CD4⁺, CD8⁺, CD33⁺, and CD19⁺ cells in the marrow were assessed by FACS.