Fig. 6.

NAC and MnTBAP prevented trauma- and hemorrhage-induced hepatic insulin resistance. WT mice were pretreated with NAC (300 mg/kg, ip) or MnTBAP (10 mg/kg, ip) before surgery. After 30 min of hemorrhage, either saline (−Ins) or 1 U insulin (+Ins) was injected into the inferior vena cava. A and B, Liver tissue lysates were subjected to Western blotting with P-Akt(S473), total (T)-Akt, and T-ERK antibodies. C and D, Autoradiographs were quantified by scanning densitometry. #, Statistics vs. TH30′+Ins; ns, not significant vs. T30′+Ins. E, Liver lysates were prepared, and aconitase activity was assayed; pretreatment with MnTBAP and NAC protected from the decrease in aconitase activity after trauma and hemorrhage. #, Statistics vs. T0′; *, statistics vs. TH30′. Data are presented as mean ± sem of samples from three to seven mice in each group. #, P < 0.05; ##, P < 0.01; ### or ***, P < 0.001.