Fig. 5. NTPDase1 modulates P2X7-dependent IL-18 release from peritoneal macrophages.
Macrophages were primed with LPS (10 ng/mL) for 3 hours and then treated for 30 min with either 1 or 2 mM ATP in the presence or absence of P2X7 antagonists (25 µM A438079 or 3 µM KN-62). The supernatants were analyzed for IL-18 by ELISA. Data show mean + SEM of n = 3–4 experiments. IL-18 release was normalized with nigericin as a stimulus; 100% release varied from 15 to 150 pg/106 cells depending on the experiment. *p<0.05, ***p<0.001, two-way ANOVA with Bonferroni post hoc test.