Table 4. Quantification of bile duct defects in larvae treated with various chemical inhibitors.

Total numbers of ducts, interconnecting ducts (IC), and terminal ductules are depicted in control, fasudil-, RKI-, dicoumeral-, colchicine-, and cytochalasin D-treated larvae. Also depicted are average duct length, width, and angles between branching ducts. Length is based on grids in Photoshop, while width is based on mm from a 5 cm × 5 cm image.

Condition	# of ducts	# IC ducts	# terminal ductules	Duct length	Duct width	Duct angles
Control	50.2±5.7	16.8±5.0	84.7±17.4	1.64±0.22	0.413±0.053	80.2±3.7
Fasudil	27.2±10.8**	3.5±3.2***	22.3±5.7***	1.11±0.27**	0.363±0.038	73.0±4.2*
RKI	28.7±11.4**	5.8±4.2**	31.2±8.4***	1.59±0.56	0.411±0.048	78.7±7.0
Dicoumeral	22.7±4.6***	0.2±0.4***	11.3±7.3***	1.14±0.35*	0.531±0.114*	78.6±6.8
Colchicine	32.0±14.8*	3.2±2.9***	15.5±11.1***	1.13±0.35*	0.411±0.076	72.0±4.4*
Cytochalasin	29.7±12.7**	1.8±1.5***	24.0±24.7**	1.20±0.35*	0.424±0.064	77.0±8.6

^*p<0.05,

^**p<0.005,

^***p<0.0005.

Values without asterisks are not significantly different from control. n=6 per condition, ±S.D.