Table 5. Quantification of bile duct defects in larvae treated with pk1a MO-injection and hnf6 MO or vhnf1 RNA injection.

Total numbers of ducts, interconnecting ducts (IC), and terminal ductules are depicted in control larvae, and larvae injected with pk1a MO, low dose (ld) pk1a MO and low dose hnf6 MO, and pk1a MO plus vhnf1 mRNA. Also depicted are average duct length, width, and angles between branching ducts. Length is based on grids in Photoshop, while width is based on mm from a 5 cm × 5 cm image.

Condition	# of ducts	# IC ducts	# terminal ductules	Duct length	Duct width	Duct angles
Control	50.2±5.7	16.8±5.0	84.7±17.4	1.64±0.22	0.413±0.053	80.2±3.7
pk1a MO	21.5±11.1***	1.3±0.8***	16.8±6.9***	1.20±0.30*	0.401±0.056	71.0±6.9*
ld pk1a MO	18.0±9.8***	0.7±0.8***	26.2±21.0***	1.34±0.53	0.374±0.010	73.6±3.4*
+ld hnf6 MO
pk1a MO +	37.7±11.0*	14.0±4.6	94.2±28.9	1.98±0.27*	0.408±0.071	73.3±4.5*
vhnf1 mRNA

^*p<0.05,

^**p<0.005,

^***p<0.0005.

Values without asterisks are not significantly different from control. n=6 per condition, ±S.D.