Table 2. Cost comparison of CUMA and TaqMan MGB probes for SNP genotyping.
| Real-time PCR (AB7900HT) | CUMA (ABI PRISM 3100) | |
|---|---|---|
| Initial start-up cost (single assay): | Price* $3,996.47 |
Price* $2,271.01 |
| SINGLEPLEX | ||
| DNA polymerase | $1.62 | $15.44 |
| Universal tails (UTs) | N/A | $3.50 |
| Unlabeled primers | $0.09 | $0.14 |
| TaqMan MGB probes | $17.28 | N/A |
| TaqMan universal PCR mastermix | $38.10 | N/A |
| PCR consumables | $9.06 | $8.11 |
| Electrophoresis consumables | N/A | $66.95 |
| Total: | $66.15 | $94.14 |
| FIVE LOCI | ||
| DNA polymerase | $6.80 | $15.44 |
| Universal tails (UTs) | N/A | $3.50 |
| Unlabeled primers | $0.46 | $0.68 |
| TaqMan MGB probes | $86.40 | N/A |
| TaqMan universal PCR mastermix | $190.50 | N/A |
| PCR consumables | $45.30 | $8.11 |
| Electrophoresis consumables | N/A | $66.95 |
| Total: | $329.46 | $94.68 |
| TWENTY LOCI | ||
| DNA polymerase | $27.20 | $61.76 |
| Universal tails (UTs) | N/A | $14.00 |
| Unlabeled primers | $1.80 | $2.70 |
| TaqMan MGB probes | $345.60 | N/A |
| TaqMan universal PCR mastermix | $762.00 | N/A |
| PCR consumables | $181.20 | $32.44 |
| Electrophoresis consumables | N/A | $66.95 |
| Total: | $1,317.80 | $177.85 |
| Redesign costs (per locus) | ||
| Unlabelled primers | $11.60 | $25-30 |
| TaqMan MGB probes | $540.00 | N/A |
| Total | $551.60 | $25-30 |
*
using 10uL PCR volumes. Cost is based on genotyping 46 samples and includes four wells (in duplicate) dedicated to positive and negative controls. Total costs assume 96-well format. All costs current as of May 2010 and inclusive of tax.