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. Author manuscript; available in PMC: 2012 Feb 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2010 Dec 9;251(1):1–7. doi: 10.1016/j.taap.2010.12.001

Fig. 1.

Fig. 1

BER activity in the splenic NEs of control and aniline-treated rats. The assay was conducted with end-labeled oligonucleotides (5-OHU:Bubble) which are targeted by NEILs (see methods for details). (A) Autoradiogram of incision products (P) generated over time by cleavage of end-labeled double-stranded oligonucleotides carrying the 5-OHU:Bubble adducts (S). Negative control (without NEs) and positive control (recombinant NEIL2) are included in the external lanes. Data represents 1 of the 5 samples assayed for BER activity in each group. (B) Values from Phosphorimager quantitation of products, generated with NEs from 5 rats per group and 3 cleavage assays per extract, were averaged and plotted as means ± SD. * Indicate s that the cleavage is significantly different from controls for each respective time point (p < 0.05).