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. 2010 Dec 15;30(50):16959–16969. doi: 10.1523/JNEUROSCI.1807-10.2010

Figure 1.

Figure 1.

Effects of LOF and GOF of dLRRK on synaptic differentiation. A, Quantification of the total number of boutons (black bar) and the number of type 1b boutons (white bar) per muscle area on muscle 6/7 of A3 in dLRRK mutant and rescued animals: WT, n = 19; dLRRKe03680, n = 54; dLRRKDf/dLRRKe03680, n = 33; muscle WT-rescue (UAS–dLRRK–WT/+; Mhc–GAL4, dLRRKe03680/dLRRKe03680), n = 30; neuronal WT-rescue (elav–GAL4/+; UAS–dLRRK–WT/+; dLRRKe03680/dLRRKe03680), n = 26; muscle 3KD-rescue (Mhc–GAL4, dLRRKe03680/dLRRKe03680, UAS–dLRRK–3KD), n = 23; neuronal 3KD-rescue (elav–GAL4/+; dLRRKe03680/dLRRKe03680, UAS–dLRRK–3KD), n = 20). Data represent values relative to control. Note that the rescuing effect of postsynaptic dLRRK is significantly different from dLRRK–3KD when directly compared. B, C, Quantification of the total number of boutons (black bar) and the number of type 1b boutons (white bar) on muscle 6/7 of A3, after normalization with muscle area, in dLRRK overexpression animals of the indicated genotypes: Mhc–GAL4/+, n = 23; Mhc–GAL4>UAS–dLRRK–WT/UAS–dLRRK–WT, n = 36; Mhc–GAL4>UAS–dLRRK–I1915T/UAS–dLRRK-I1915T, n = 35; elav–GAL4/+, n = 31; elav–GAL4>UAS–dLRRK–WT/UAS–dLRRK–WT, n = 30; elav–GAL4>UAS–dLRRK–I1915T/UAS–dLRRK-I1915T, n = 40 (B), Mhc–GAL4/+, n = 25; Mhc–GAL4>hLRRK–WT, n = 23; Mhc–GAL4>hLRRK–G2019S, n = 23; elav–GAL4/+, n = 24; elav–GAL4>hLRRK–WT, n = 23; elav–GAL4>hLRRK–G2019S, n = 21 (C). Data represent values relative to control. D, E, HRP immunostaining showing the effects of dLRRK LOF and dLRRK or hLRRK2 GOF on synaptic morphology. NMJs of wild-type, dLRRK mutant, and dLRRK mutant with neuronal or muscle rescue (D) or animals overexpressing dLRRK–WT, dLRRK–3KD, or hLRRK2 specifically in muscle or neurons (E) are shown. Genotypes shown are as follows: dLRRK–3KD muscle overexpression (genotype, UAS-dLRRK–3KD; Mhc–GAL4), dLRRK–WT muscle overexpression (genotype, UAS–dLRRK–WT/+; UAS–dLRRK–WT/Mhc–GAL4), hLRRK2 muscle overexpression (genotype, Mhc–GAL4/UAS–hLRRK2–WT–Flag), dLRRK–3KD neuronal overexpression (genotype, elav–GAL4; UAS–dLRRK–3KD), dLRRK–WT neuronal overexpression (genotype, elav–GAL4/+; UAS–dLRRK–WT/+; UAS–dLRRK–WT/+), and hLRRK2 neuronal overexpression (genotype, elav–GAL4/+; UAS–hLRRK2–WT–Flag/+). Scale bars, 20 μm.