Figure 8.

PpHxk3 can complement a hexokinase-deficient yeast strain. The picture shows growth of the hxk1 hxk2 glk1 triple disrupted yeast strain containing the pFL61 vector with different inserts on plates containing 2% galactose, 2% glucose or 3% raffinose as carbon source. Growth on glucose requires glucokinase activity and growth on raffinose fructokinase activity. The inserts from left to right are: PpHXK3 cDNA; PpHXK1 cDNA encoding an N-terminally truncated protein; PpHXK1 cDNA; no insert.