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. Author manuscript; available in PMC: 2011 Feb 28.
Published in final edited form as: Anal Biochem. 2008 Sep 14;384(1):136–144. doi: 10.1016/j.ab.2008.09.006

Fig. 6.

Fig. 6

MS analysis of the sample from fraction 13 shown in Fig. 5. (A) The mass of one ion with m/z 1469.8 matches the calculated mass of the peptide 509NQIAACLPTR518 with one molecule of MDCC (MH+ of 1469.7). (B) MS/MS spectrum of the precursor ion with m/z 1469.8 and assignment of the peaks to b ions and y ions calculated from peptide 509NQIAACLPTR518, which contains MDCC modification at C514. The arrows under the abscissa show locations of ions anticipated to arise from the alternate labeling sites (Table 3) but not seen in the MS. The hypothesized fragmentation site of MDCC is indicated by a dashed line, and the calculated mass of the ion generated from the left portion of MDCC is 244.1 Da.