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. Author manuscript; available in PMC: 2011 Feb 28.
Published in final edited form as: Biol Reprod. 2008 Feb 14;78(6):1064–1072. doi: 10.1095/biolreprod.107.063743

FIG. 8.

FIG. 8

Effects of E2, MPA, IL1B, and TNF on MMP2 and MMP9 mRNA levels in first-trimester decidual cell monolayers. Confluent passaged, leukocyte-free decidual cells were incubated for 7 days in 10−8 M E2 + 10−7 M MPA, then switched to DM with E2 + 10−7 M MPA alone or with IL1B or TNF for 6 h. Quantitative real-time RT-PCR was performed on extracted RNA for MMP2 and MMP9 normalized to ACTB mRNA (n = 4; mean ± SEM). P < 0.05 *versus E2 or E2 + MPA; **versus corresponding MMP2 fold change. A) MMP2; linear plot: ordinate RNA/ACTB mRNA. B) MMP9; linear plot: ordinate RNA/ACTB mRNA. C) MMP2 and MMP9; semi log plot: ordinate fold change from E2.

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