FIG. 9.

Effects of E₂, MPA, IL1B, and TNF on TIMP1 (top panel) and TIMP2 (bottom panel) output by first-trimester decidual cell monolayers. Confluent, leukocyte-free decidual cells were incubated for 7 days in 10⁻⁸ M E₂, or E₂ + 10⁻⁷ M MPA, then switched to DM with corresponding steroid(s) with or without 1 ng/ml of IL1B or TNF for 24 h. Secreted levels of TIMP1 and TIMP2 were measured by ELISA in conditioned DM and normalized to cell protein (n = 8; mean ± SEM). No significance was found when comparing baseline to either cytokine for either TIMP1 or TIMP2.