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. Author manuscript; available in PMC: 2011 Feb 28.
Published in final edited form as: Biotechniques. 2007 Jun;42(6):732–737. doi: 10.2144/000112451

Figure 2. Immunohistochemically stained sections of centrifuged (indicated as C) and uncentrifuged (indicated as U) aggregates.

Figure 2

Immunoreactivity for collagen types I, II, and X was detected using a fluorescein isothiocyanate (FITC)-labeled secondary (green) antibody; 4′,6-diamidino-2-phenyl-indole (DAPI) counter-stain of the nuclei (blue). Scale bar, 1 mm.