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. Author manuscript; available in PMC: 2012 Jan 20.
Published in final edited form as: Virology. 2010 Nov 5;409(2):262–270. doi: 10.1016/j.virol.2010.10.016

Figure 1. Expression and purification of recombinant MBP-Oas1 proteins.

Figure 1

(A) MBP-Oas1 proteins were expressed in bacteria, partially purified on amylose columns, separated by 10% SDS-PAGE and visualized by Coomassie blue staining. Lane 1, MBP-Oas1a. Lane 2, MBP-Oas1b. Lane 3, MBP-Oas1btr. Asterisks indicate the positions of the MBP-Oas1 fusion proteins. (B) Immunoblot of partially purified MBP-Oas1 proteins detected with anti-MBP-HRP antibody. Lane 1, MBP-Oas1a. Lane 2, MBP-Oas1b. Lane 3, MBP-Oas1btr. Lane 4, MBP. (C) Immunoblot of partially purified MBP-Oas1b protein detected with anti-OAS1 antibody.