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. Author manuscript; available in PMC: 2012 Jan 20.
Published in final edited form as: Virology. 2010 Nov 5;409(2):262–270. doi: 10.1016/j.virol.2010.10.016

Figure 2. Analysis of MBP-Oas1 protein 2-5A synthetase activity.

Figure 2

Different amounts of MBP-Oas1 proteins were incubated with α32p-ATP and poly (I:C) for 18 hrs at 30°C. Four μl (A) or two μl (B and C) of each reaction were then electrophoresed on a 20% polyacrylamide urea denaturing gel. (A) Analysis of MBP-Oas1a 2-5A synthetase activity. Reactions contained: Lane 1, No protein. Lane 2, 16 μg of MBP. Lane 3, 0.75 μg of Oas1a. Lanes 4-7, 0.5 μg, 0.75 μg, 1 μg, and 16 μg of MBP-Oas1a, respectively. (B) Analysis of MBP-Oas1b 2′-5′ Oas activity. Reactions contained: Lane 1, No protein. Lane 2, 2.3 μg of MBP-Oas1btr. Lane 3, 2.6 μg of MBP-Oas1b. Lanes 4-6, 1 μg, 0.75 μg, and 0.5 μg of MBP-Oas1a, respectively. (C) Analysis of the effect of poly(I:C) on MBP-Oas1b synthetase activity. Reactions contained: Lane 1, No protein. Lane 2, 1.5 μg of MBP-Oas1b without poly(I:C). Lane 3, 1.5 μg of MBP-Oas1b with 50 μg/ml poly(I:C). Lane 4, 0.5 μg of MBP-Oas1a with poly(I:C). The data shown are representative of results obtained from at least three repeats using different pooled protein preparations.