Figure 3. Analysis of Oas1 protein dsRNA binding activity.
The ability of MBP-Oas1a, MBP-Oas1b or MBP to bind dsRNA poly(I:C)-agarose was tested. Bound RNA-protein complexes were separated by 10% SDS-PAGE. Immunoblotting was performed using anti-MBP antibody. Lys, lysate; pIC, poly(I:C)-bound fraction; Unb, unbound fraction. The data shown are representative of results obtained from at least three repeats.