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. 2011 Mar 1;22(5):581–592. doi: 10.1091/mbc.E10-06-0537

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© 2011 Gentile et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — RT-PCR analysis for skeletal muscle markers of human cells cocultured with mouse myoblasts. Human cells were cultured for 48 h in DM in the absence or presence (+) of differentiating mouse primary myoblasts. mRNA expression was evaluated by RT-PCR analysis. With the exception of β-actin (β-act) in lane 2, primers were selected so to be specific for human transcripts only. MYO: human skeletal muscle positive control; ENDO: endometrium-derived endothelial cells; Myog: Myogenin; CHRNA1: cholinergic nicotinic receptor A1; MYH2: adult skeletal myosin heavy chain.