FIGURE 1:

Cdc42p is depolarized in the sec10 mutant but remains polarized in the exo70 mutant cells. (A) Immunofluorescence staining of Cdc42p in wild-type (WT), sec10-2, and exo70-38 cells. Cells were grown to log phase at 25°C and shifted to 37°C for 120 min, fixed, and immunostained for Cdc42p. Bar = 10 μm. (B) Quantification of Cdc42p polarization in WT, sec10-2, and exo70-38 cells. Fifty small-budded cells were counted for each group (n = 3). Error bars represent standard error. *, p < 0.05. (C) WT and exo70-35 cells were grown to log phase at 25°C, fixed, and immunostained for Cdc42p. Bar = 10 μm. (D) Quantification of Cdc42p polarization in the WT and exo70-35 cells. Fifty cells were counted for each group (n = 3).