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. 2011 Mar 1;22(5):687–702. doi: 10.1091/mbc.E10-07-0632

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© 2011 M. D. Leach et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — Many C. albicans proteins are sumoylated. Cells expressing FLAG-Smt3 (MLC01) were grown for 5 h and then stressed for 1 h with the appropriate stress at the concentrations indicated in Materials and Methods. The parental C. albicans strain (THE1) was used for the no-FLAG control. Protein extracts were prepared and analyzed by Western blotting with an α-FLAG antibody. The band presumed to be FLAG-Smt3 is highlighted.