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. 2011 Mar 1;22(5):687–702. doi: 10.1091/mbc.E10-07-0632

FIGURE 9:

FIGURE 9:

Mutation of sumoylation sites in Hsp104 and Hsp60 mimics phenotypes of the C. albicans smt3 null mutant. (A) Sensitivity of an Hsp104 sumoylation mutant to a 30–42ºC heat shock. Cell viability was assayed 30 and 60 min after the heat shock: HSP104/HSP104 (MLC43, open circles), hsp104/HSP104 (MLC56, gray triangles), and hsp104/HSP104-K356R (MLC59, black squares). (B) Light microscopy of a C. albicans Hsp60 sumoylation mutant grown at 30ºC reveals their abnormal morphology: hsp60/HSP60 (MLC52) and hsp60/HSP60-K324R (MLC55). (C) Sensitivity of the Hsp60 sumoylation mutant to a 30–42ºC heat shock in the presence of a respiratory inhibitor. Cells were grown in the presence of 1 μg/ml antimycin A and then subjected to a 42ºC heat shock for 30 min: HSP60/HSP60 (MLC43), hsp60/HSP60 (MLC52), and hsp60/HSP60-K324R (MLC55). **p < 0.01 (Student’s t test). These strains showed no difference in heat shock sensitivity in the absence of antimycin A (unpublished data).