Fig. 3.

Altered hematopoiesis in induced SHP-2-deficient mice. (A) Numbers of total thymocytes (left) and indicated thymocyte subpopulations (right) in thymi isolated from tamoxifen-injected moribund ptpn11^fl/fl ert2-cre mice (n=8) and littermate controls (n=8). DN, CD4–CD8–double negative; DP, CD4+CD8+ double positive; SP, CD4+CD8– and CD4–CD8+ single positive T cells. (B) Numbers of total splenocytes (left) and indicated splenocyte subpopulations (right) in spleens isolated from tamoxifen-injected moribund ptpn11^fl/fl ert2-cre mice (n=12) and littermate controls (n=14). T cells (T), B cells (B), macrophages (Mac) and neutrophils (Neut) were identified as TCRβ+, B220+, CD11b^int/GR1^int and CD11b^hi/GR1^hi, respectively. Ter119+ cells represent erythrocyte precursors. (C) Total numbers and numbers of indicated lineage-positive cells (left) and numbers of indicated lineage-negative progenitor cells (right) in bone marrow of tamoxifen-injected moribund ptpn11^fl/fl ert2-cre mice and littermate controls (for each genotype, n=11 for lineage-positive and n=5 for lineage-negative). LSK, Lin⁻Sca-1⁺c-Kit⁺ cells, which are enriched for HSCs; CLP, Lin⁻Sca-1^loc-Kit^loCD127⁺; CMP, Lin⁻Sca-1⁻c-Kit⁺CD34⁺CD16/32^lo; GMP, Lin⁻Sca-1⁻c-Kit⁺CD34⁺CD16/32^hi; MEP, Lin⁻Sca-1⁻c-Kit⁺CD34⁻CD16/32^–/lo. In scatter plots, each symbol represents an individual mouse. In bar graphs, the mean ± 1 s.e.m. is depicted. All mice were injected with tamoxifen at 6–8 weeks of age. Age range of mice at time of analysis was 10–16 weeks. Statistical significance was determined by two sample Student’s t-test. *P<0.05; **P<0.005.