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. 2011 Mar;4(2):268–276. doi: 10.1242/dmm.002998

Fig. 1.

Fig. 1.

The BAC NEUROG3-SeAP/EGFP transgenes are expressed in the developing pancreas. (A) Homologous recombination (Yang et al., 1997) was used to replace the human neurogenin-3 coding sequence in the NEUROG3 BAC (RP11-343J3T) with two reporter genes, SeAP and EGFP, flanked on the 5′ end by the human β-globulin intron and the 3′ end by the SV40 polyadenylation signal, and separated by a viral IRES. (B) Levels of neurogenin-3 mRNA in mouse pancreas were measured by real-time TaqMan RT-PCR at the embryonic dates shown and in adult islets, and are expressed relative to levels of histone H3.3a mRNA. (C) Levels of the SeAP/EGFP mRNA in mouse pancreas were measured by real-time SYBR Green RT-PCR at the embryonic dates shown and are expressed relative to levels of mouse β-actin mRNA. (D) Tissue SeAP activity was measured in pancreas homogenates at the embryonic dates shown and is expressed relative to total protein. All data represent mean + s.e.m. from at least three independent experiments.