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. 2011 Mar;4(2):268–276. doi: 10.1242/dmm.002998

Fig. 2.

Fig. 2.

The EGFP transgene is expressed in the endocrine lineage in the embryonic pancreas of BAC NEUROG3-SeAP/EGFP transgenic mice. (A,B) Native EGFP fluorescence from whole-mounted pancreas at E15.5 (A) and E16.5 (B). (C) Immunofluorescent staining of whole-mounted E15.5 pancreas for mucin-1 (blue) together with immunofluorescent staining for EGFP (green). (D–I) Immunofluorescent staining of frozen E15.5 pancreatic sections for EGFP (green) and neurogenin-3 (red) together with glucagon (blue; D and E) or insulin (blue; G and H). White arrowheads mark representative EGFP positive cells co-stained with neurogenin-3. Yellow arrowheads mark representative EGFP-positive cells co-stained with glucagon or insulin. Scale bars: 50 μm.