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. 2011 Feb 28;6(2):e17145. doi: 10.1371/journal.pone.0017145

Figure 1. Identification of Z5463PI as the infectious strain.

Figure 1

A- Pulsed-Field Gel Electrophoresis (PGFE) analysis using the enzyme BglII for DNA digestions, revealed by ethidium bromide. B- Southern-blot on the PFGE gel using a probe against a MDA gene (NMA1792). The Southern-blot is showing a second insertion of the MDA in Z5463PI and Z5463BC. M: molecular weight, 1: Z5463, 2: Z5463PI, 3: Z5463BC. C- Schematic presentation of the different insertions of the MDA in Z5463PI and Z5463BC when compared to Z2491 and Z5463. 1. Locus of the wild type insertion of the MDA (bases 1 737 566 to 1 742 107 of Z2491 genome), 2. Locus corresponding to the base 1 058 420 to 1 059 338 of Z2491, containing a second insertion of the MDA in Z5463PI and Z5463BC in a dRS3 target sequence containing a dinucleotide CT at position 1058887 of Z2491 genome.