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. 2011 Feb 28;6(2):e16687. doi: 10.1371/journal.pone.0016687

Figure 1. RNA binding activities eluted from the dengue 3′SL RNA columns.

Figure 1

S10 extract from K562 cells was prepared and chromatographed as described in the Materials and Methods section. In both panels, lane 1 represents the dengue 3′SL RNA only. The second lane shows the binding properties of proteins that washed through the column in the flow through (FT) fraction. The five column washes are analyzed in lanes 3–7. Bound proteins were step-eluted with 250 mM, 500 mM, 1 M and 2 M NaCl (lanes 8–11 respectively). (A) The S10 extract was pre-cleared by passing it over the control (lacking bound RNA) column. (B) The pre-cleared extract was chromatographed on the dengue 3′SL affinity column. The EMSA using the fractions eluted from the RNA affinity column shows three binding activities eluted from the column, RNP1, RNP2, and RNP3 (lanes 9–11).