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. 2011 Feb 28;6(2):e16687. doi: 10.1371/journal.pone.0016687

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Gomila et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Open arrows point to representative uninfected cells; filled arrows point to representative cells infected by dengue virus. (A) Cells were stained with the nuclear stain DAPI, with anti-NF90 mouse monoclonal antibody, and anti-dengue virus NS3 rabbit polyclonal antibodies. The merge panel shows an overlay of the DAPI, NF90 and NS3 signals. (B) Cells were stained with the nuclear stain DAPI, with anti-RHA rabbit polyclonal antibodies, and anti-dengue prM mouse monoclonal antibody. The merge panel shows an overlay of the DAPI, RHA and prM signals. Different dengue antibodies were used in panels A and B because of the requirement for secondary antibody specificities.