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. 2011 Feb 28;6(2):e16687. doi: 10.1371/journal.pone.0016687

Figure 5. Supershift assay confirms the presence of NF90 bound to dengue 3′ SL RNA.

Figure 5

A bandshift assay, as described for Figure 1, was performed after incubating radiolabeled RNA and proteins extracts in the absence of added antibody (lanes 1, 4, 7, 10), in the presence of anti-NF90 antibody (lanes 2, 5, 8, 11), or in the presence of anti-eEF1A antibody (lanes 3, 6, 9, 12). All reactions included the same amount of radiolabeled dengue 3′ SL RNA. Lanes 1–3: RNA only incubated in the absence (lane 1) or presence (lanes 2, 3) of indicated antibody. Lanes 4–6 represent bandshift assays using S10 extract from K562 cells in the absence/presence of the indicated antibody. Lanes 7–9 represent bandshift assays using the 500 mM fraction as protein source, in the absence/presence of the indicated antibody. An asterisk (lane 7) has been placed immediately above the RNP2 band previously identified in Figure 1B. Lanes 10–12 represent bandshifts assay using the 1 M fraction as a protein source, in the absence/presence of the indicated antibody.