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. 2011 Feb 28;6(2):e16687. doi: 10.1371/journal.pone.0016687

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Gomila et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Supernatants from WT and NF90-depleted cells infected with dengue virus (multiplicity of infection: 0.5) were used to infect DC-SIGN-expressing K562 cells (12, 46). At 14 hours post-infection, cells were harvested and analyzed for viral infection by flow cytometry using antibodies against dengue virus prM. Panels A-D are representative dot plots from the assay of a single set of supernatants from WT (MDA-MB-435-GFP) and KD (shDRBP76-GFP cells), FSC: forward scattering. (E) Bar graph summarizing the results of three independent experiments. Asterisks represent statistical significance at P<0.02.