Table 1. Summary of MALDI-TOF MS results from the excised protein bands.
Band | MOWSEScorea | MassesMatchedb | Coveragec | MW (Da) | Protein |
90 kDa | 115 | 100% | 22% | 74560 | NF90 |
140 kDa | 84 | 84% | 13% | 140869 | RHA |
42 kDa | 182 | 89% | 38% | 44669 | NF45 |
The MOWSE score reflects the probability that the submitted masses are not a random match; scores greater than 76 (P<0.05) are considered significant.
Number of masses that were matched with predicted peptides with high probability.
Coverage refers to the percentage of the protein that was spanned by the predicted peptides.