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. 2011 Feb 28;6(2):e17023. doi: 10.1371/journal.pone.0017023

Figure 2. RXR-γ levels in SCs are induced by axonal contact mimickers.

Figure 2

(A–D) Isolated SCs were adapted for 24 hours to the absence of the axonal contact mimickers BPE and forskolin and subsequently treated for 48 hours with 2 µM forskolin (Fk), 60 µg/ml BPE or the combination of both, and the levels of RXR-γ (A), RAR-β (B), MAG (C) and Egr2 (D) were analyzed by Q-RT-PCR. In all instances, the levels reached after the acute combined treatment were compared to SCs continuously grown in the presence of BPE and Fk (BPE+Fk always). (E–F) SCs were adapted for 24 hours to the absence the axonal contact mimickers and subsequently treated for 48 hours with 2 µM forskolin (Fk), 5 µM cyclopamine (CPM) or the combination of both, and the levels of RXR-γ (E) and RAR-β (F) were analyzed by Q-RT-PCR. All values are shown as the mean ± SD relative to their respective controls (cells grown in the presence of vehicle). Significance was assessed by unpaired Student's t-test. n.s.: non-significant; *: p<0.05; **: p<0.01; ***: p<0.005.