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. 2011 Feb 28;6(2):e16722. doi: 10.1371/journal.pone.0016722

Figure 6. Increased VEGF-to-PEDF ratio in human RPE cells following repetitive oxidative injury with HQ.

Figure 6

Confluent serum-starved ARPE-19 cells were treated with 50 µM HQ every 4 days for 24 hours for 3 consecutive weeks in phenol red-free 0.1% FBS medium. Total RNA was extracted to assess (A) VEGF and (D) PEDF mRNA expression by real-time PCR. GAPDH was used as internal control. Supernatants were collected to assess (B) VEGF and (D) PEDF protein concentration by ELISA. (E) VEGF-to-PEDF protein ratio. Data are mean± SE and represent the average results of 3 independent experiments run in duplicate. * is p<0.05 and *** is p<0.0001 versus control.