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. 2011 Feb 28;6(2):e17321. doi: 10.1371/journal.pone.0017321

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Capsoni et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Western blot and densitometric analysis of (A) TrkA (Y490), (B) Akt/S473), and (C) PLC-γ1 (Y783) phosphorylation, in extracts from BALB/C 3T3 TrkA cells, stimulated by 100 ng/ml of hNGF and hNGFR100 mutants. D, Western blot of phospho-TrkA (Y490), Akt (S473), PLC-γ1 (Y783) and Erks (T204/Y202) in PC12 cells, stimulated by 5 ng/ml of hNGF and hNGFR100E mutant. E, Densitometric analysis of phospho-TrkA. F, Densitometric analysis of phospho-Akt. G, Densitometric analysis of phospho-PLC-γ1. H, Densitometric analysis of phospho-Erks. I, Densitometric analysis of phospho c-jun in hippocampal neurons. The experiments were performed in triplicate. Bars represent the mean ± s.e.m.